CD40 antibody

Catalog No. ABIN1804378

This Mouse Monoclonal antibody specifically detects CD40 in FACS, IHC, IP, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD40 antibody (ABIN1804378)

Target: CD40

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD40 Antibody

Brand: IHC-plus™

Specificity: Recognizes human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions.

Purification: Protein A purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (20 μg/mL), ICC (1:10), IHC, IHC-Fr, IHC-P (10 μg/mL), IP

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Flow Cytometry: 20 μg/mL. Use 10 μL to lane 1 x10⁶ in 100 μL. Immunohistochemistry (Frozen and paraffin): 1-10 μg/mL. Requires protein digestion pre-treatment of paraffin embedded sections prior to staining. Immunocytochemistry: 1:10 μg/mL. Positive control: Human tonsil.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: TBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: Name/Gene ID: CD40
Family: TNF Receptor

Synonyms: CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5

Gene ID: 958

UniProt: P25942

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints