PDCD1LG2 antibody

Catalog No. ABIN181078

This Rat Monoclonal antibody specifically detects PDCD1LG2 in FACS, IP and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for PDCD1LG2 antibody (ABIN181078)

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PDCD1LG2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: TY25

Product Details anti-PDCD1LG2 Antibody

Specificity: This antiobdy recognises mouse CD273, a 42 kDa member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2).

Characteristics: Synonyms: PD-L2, PD-1-ligand 2, PDCD1 ligand 2, PDCD1LG2, B7DC, PDCD1L2, Programmed cell death 1ligand 2, Programmed death ligand 2

Purification: Affinity chromatography on Protein G

Immunogen: B7-DC transfected RAW264. 7 cells. Lymph node cells from immunised SD rats were fusedwith the cells of the P3U1 myeloma cell line. Genename: CD273

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/100 - 1/200, use 10 μL of the suggested working dilution to label 10^6cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PDCD1LG2

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: CD273 / PDL2

Background: The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD279). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Antibodies produced by clone TY25 are reported to block the binding of CD273 (B7-DC) to it's receptor.Synonyms: B7DC, PD-1-ligand 2, PD-L2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, Programmed cell death 1 ligand 2, Programmed death ligand 2

Gene ID: 58205

UniProt: Q9WUL5