CD79a antibody (AA 202-216)

Catalog No. ABIN181100

The Mouse Monoclonal anti-CD79a antibody has been validated for FACS, WB, IHC (p) and IHC (fro). It is suitable to detect CD79a in samples from Human, Mouse, Rat, Cow, Pig, Monkey, Chicken, Rabbit, Horse, Guinea Pig and Dog.

Quick Overview for CD79a antibody (AA 202-216) (ABIN181100)

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reactivity: Human, Mouse, Rat, Cow, Pig, Monkey, Chicken, Rabbit, Horse, Guinea Pig, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD79a antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HM57

Product Details anti-CD79a Antibody

Binding Specificity: AA 202-216

Specificity: This antibody recognizes an epitope within the cytoplasmic domain of CD79a.

Cross-Reactivity (Details): Species reactivity (tested):Human, American Bison, Fallow Deer, Canine, Monkey, Pig, Horse, Bovine, Guinea Pig, Rat, Mouse, Rabbit, Chicken.

Characteristics: Synonyms: IGA, MB1, B-Cell marker, B-cell antigen receptor complex-associated protein alpha-chain,MB-1 membrane glycoprotein, Surface IgM-associated protein, Membrane-boundimmunoglobulin-associated protein

Purification: Affinity Chromatography on Protein G

Immunogen: Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1. Spleencells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line. Genename: CD79a

Isotype: IgG1

Application Details

Application Notes: Western Blot. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires antigen retrieval usingheat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 isrecommended for this purpose. Recommended Positive Control: Human tonsilFlow Cytometry: 1/10 - 1/25, use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Alternative Name: CD79a

Background: CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin.Synonyms: B-Cell marker, B-cell antigen receptor complex-associated protein alpha-chain, IGA, MB-1 membrane glycoprotein, MB1, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein

Gene ID: 973

UniProt: P11912

Pathways: BCR Signaling