Nerve Growth Factor antibody

Catalog No. ABIN181153

This Mouse Monoclonal antibody specifically detects Nerve Growth Factor in WB, Func and EIA. It exhibits reactivity toward Human.

Quick Overview for Nerve Growth Factor antibody (ABIN181153)

Target: Nerve Growth Factor (NGF)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Product Details anti-Nerve Growth Factor Antibody

Specificity: This antibody detects NGF-beta.

Characteristics: Synonyms: Beta-NGF, NGFB, Beta-nerve growth factor

Purification: Affinity Chromatography on Protein G

Immunogen: Highly pure (>98%) recombinant Human beta-NGF

Isotype: IgG2b

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman beta-NGF (10.0 ng/mL), a concentration of 2.7-4.0 μg/mL of this antibody isrequired. ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 200 pg/mL of recombinant Human Beta-NGF when used withBiotin anti-Human beta-NGF antibody (cat. PP1057B) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can beused at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human beta-NGF is 4.0-8.0 ng/lane, underreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background: Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB

Gene ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size