Follistatin antibody

Catalog No. ABIN181535

The Rabbit Polyclonal anti-Follistatin antibody has been validated for WB and EIA. It is suitable to detect Follistatin in samples from Human.

Quick Overview for Follistatin antibody (ABIN181535)

Target: Follistatin (FST)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Follistatin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Follistatin Antibody

Specificity: This antibody detetcs Follistatin.

Purification: Affinity purified

Immunogen: Highly pure (> 98 %) recombinant human Follistatin

Application Details

Application Notes: ELISA: Direct: To detect hFollistatin by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin. Sandwich: To detect hFollistatin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumanFollistatin as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hFollistatin. Western Blot: To detect hFollistatin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for Follistatin

Target: Follistatin (FST)

Alternative Name: Follistatin

Background: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone. There are three different isoforms, generated by alternative splicing.Synonyms: Activin-binding protein, FS, FST

Gene ID: 10468

NCBI Accession: NP_006341

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion