BMP7 antibody (Biotin)

Catalog No. ABIN181549

This Rabbit Polyclonal antibody specifically detects BMP7 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for BMP7 antibody (Biotin) (ABIN181549)

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP7 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BMP7 Antibody (Biotin)

Specificity: This antibody detects BMP7.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human BMP-7

Application Details

Application Notes: ELISA: Direct: To detect BMP-7 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant BMP-7. Sandwich: To detect BMP-7 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Human BMP-7 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant BMP-7. Western Blot: To detect BMP-7 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant BMP-7 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Alternative Name: BMP7

Background: Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.Synonyms: BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1

Gene ID: 655

NCBI Accession: NP_001710

UniProt: P18075

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance