FGF17 antibody

Catalog No. ABIN181584

The Rabbit Polyclonal anti-FGF17 antibody has been validated for WB, EIA and Func. It is suitable to detect FGF17 in samples from Human.

Quick Overview for FGF17 antibody (ABIN181584)

Target: FGF17 (Fibroblast Growth Factor 17 (FGF17))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF17 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-FGF17 Antibody

Specificity: This antibody detects Fibroblast Growth Factor 17.

Purification: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human FGF-17

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-17 (1.5 ng/mL), a concentration of 0.020 - 0.035 μg/mL of this antibody is required. ELISA: Indirect: To detect hFGF-17 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17. Sandwich: To detect hFGF-17 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. This In conjunction with BiotinylatedAnti-Human FGF-17 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17. Western Blot: To detect hFGF-17 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-17 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for FGF17

Target: FGF17 (Fibroblast Growth Factor 17 (FGF17))

Alternative Name: FGF17

Background: FGF17 belongs to the heparin-binding growth factors family. It may be a signaling molecule in the induction and patterning of the embryonic brain and is preferentially expressed in the embryonic brain. There are two named isoforms.Synonyms: FGF-17, Fibroblast growth factor 17

Gene ID: 8822

NCBI Accession: NP_003858

UniProt: O60258

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway