IL-19 antibody (Biotin)

Catalog No. ABIN181613

The Rabbit Polyclonal anti-IL-19 antibody has been validated for WB and EIA. It is suitable to detect IL-19 in samples from Human.

Quick Overview for IL-19 antibody (Biotin) (ABIN181613)

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-19 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-19 Antibody (Biotin)

Specificity: This antibody detects Interleukin-19.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human IL-19

Application Details

Application Notes: ELISA: Direct: To detect hIL-19 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-19. Sandwich: To detect hIL-19 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Human IL-19 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant hIL-19. Western Blot: To detect hIL-19 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-19 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for IL-19

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Alternative Name: Interleukin-19 / IL19

Background: IL19 is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Little is known about the biologic function and gene regulation of IL19. Alternatively spliced transcript variants encoding distinct isoforms have been described.Synonyms: IL-19, Melanoma differentiation-associated protein-like protein, NG.1, ZMDA

Gene ID: 29949

NCBI Accession: NP_037503

UniProt: Q9UHD0