RETNLB antibody (Biotin)

Catalog No. ABIN181633

This Rabbit Polyclonal antibody specifically detects RETNLB in WB and EIA. It exhibits reactivity toward Mouse.

Quick Overview for RETNLB antibody (Biotin) (ABIN181633)

Target: RETNLB (Resistin Like beta (RETNLB))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RETNLB antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RETNLB Antibody (Biotin)

Specificity: This antibody detects Mouse RELM beta. Other species not tested.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant murine RELMbeta

Application Details

Application Notes: ELISA: Direct: To detect mRELMβ (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMβ. Sandwich: To detect mRELMβ (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-MurineRELMβ as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mRELMβ. Western Blot: To detect mRELMβ by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mRELMβ is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for RETNLB

Target: RETNLB (Resistin Like beta (RETNLB))

Alternative Name: RELM beta

Background: Synonyms: CCRG, Colon and small intestine-specific cysteine-rich protein, Colon carcinoma-related gene protein, Cysteine-rich secreted protein A12-alpha-like 1, Cysteine-rich secreted protein FIZZ2, FIZZ2, HXCP2, RELM-beta RETNLB, RELMbeta, RETNL2, Resistin-like beta

Gene ID: 57263

NCBI Accession: NP_076370

UniProt: Q99P86

Pathways: Hormone Activity