PLGF antibody (Biotin)

Catalog No. ABIN181637

The Rabbit Polyclonal anti-PLGF antibody is suitable to detect PLGF in samples from Human. It has been validated for WB and EIA.

Quick Overview for PLGF antibody (Biotin) (ABIN181637)

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLGF antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PLGF Antibody (Biotin)

Specificity: This antibody detects PlGF.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant hPlGF

Application Details

Application Notes: ELISA: Direct: To detect hPlGF (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF. Sandwich: To detect hPlGF (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanPlGF as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hPlGF. Western blot: To detect hPlGF this antibody can be used at a concentration of 0.1 - 0.2g/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hPlGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for PLGF

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Alternative Name: Placenta Growth Factor / PGF

Background: Synonyms: PGFL, PLGF, PlGF

Gene ID: 5228

NCBI Accession: NP_001193941

UniProt: P49763

Pathways: VEGFR1 Specific Signals