ARTN antibody

Catalog No. ABIN181680

The Rabbit Polyclonal anti-ARTN antibody has been validated for WB and EIA. It is suitable to detect ARTN in samples from Human.

Quick Overview for ARTN antibody (ABIN181680)

Target: ARTN (Artemin (ARTN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARTN antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ARTN Antibody

Specificity: This antibody detects Artemin.

Purification: Immunoaffinity Chromatography

Immunogen: Highly pure (> 98%) derived recombinant Human Artemin

Application Details

Application Notes: ELISA: Indirect: To detect hArtemin (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hArtemin. Sandwich: To detect hArtemin (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanArtemin as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hArtemin. Western blot: To detect hArtemin this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for ARTN

Target: ARTN (Artemin (ARTN))

Alternative Name: Artemin

Background: Artemin is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands, a group of ligands within the TGF-beta superfamily of signalling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenrative disease. Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Four alternatively spliced transcripts have been described, two of which encode the same protein.Synonyms: ARTN, EVN, Enovin, Neublastin

Gene ID: 9048

NCBI Accession: NP_001129687

UniProt: Q5T4W7