Galectin 3 antibody (Biotin)

Catalog No. ABIN181681

This Rabbit Polyclonal antibody specifically detects Galectin 3 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Galectin 3 antibody (Biotin) (ABIN181681)

Target: Galectin 3 (LGALS3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Galectin 3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Galectin 3 Antibody (Biotin)

Purification: Affinity Chromatography.

Immunogen: Highly pure (> 98%) recombinant human Galectin-3

Application Details

Application Notes: ELISADirect: To detect hGalectin-3 (using 100 μL/well antibody solution) a concentration ofapprox. 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanGalectin-3. Sandwich: To detect Human Galectin-3 (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. In conjunction with PolyclonalAnti-Human Galectin-3 (ABIN181683 or ABIN181684) as a capture antibody, it allows thedetection of at least 0.2-0.4 ng/well of recombinant Human Galectin-3. Western blot

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: Galectin-3

Target Type: Chemical

Background: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin-3 regulates a number of biological processes, including embryogenesis, inflammatory responses, cell progression and metastasis. Galectin-3 is normally expressed in epithelia of a variety of tissues, including colon and endometrium, and in various inflammatory cells, including macrophages. Galectin-3 can function intracellularly, in controlling cell cycle and preventing T-cell apoptosis, and also extracellularly, in activating various cells, including monocytes/macrophages, mast cells, neutrophils, and lymphocytes. Expression of Galectin-3 is affected by neoplastic transformation, being up-regulated in certain types of lymphomas, and in thyroid and hepatic carcinomas. Conversely, it is down-regulated in other cancers, such as colon, breast, ovarian, and uterine.Synonyms: 35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBP, Galactose-specific lectin 3, IgE-binding protein, L-31, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Lgals3, Mac-2

Gene ID: 9606

UniProt: P17931

Pathways: RTK Signaling