IL17B antibody (Biotin)

Catalog No. ABIN181689

This anti-IL17B antibody is a Rabbit Polyclonal antibody detecting IL17B in WB and EIA. Suitable for Human.

Quick Overview for IL17B antibody (Biotin) (ABIN181689)

Target: IL17B (Interleukin 17B (IL17B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL17B antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL17B Antibody (Biotin)

Specificity: This antibody detects Human Interleukin-17 beta. Other species not tested.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human IL-17 beta

Application Details

Application Notes: ELISA: Direct: To detect hIL-17B (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17B. Sandwich: To detect hIL-17B (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanIL-17B as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-17B. Western blot: To detect hIL-17B this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIL-17B is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for IL17B

Target: IL17B (Interleukin 17B (IL17B))

Alternative Name: Interleukin-17B / IL17B

Background: IL17B is a non disulfide-linked homodimer of two 161 amino acid polypeptide chains. It belongs to the IL17 family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL17A through IL17F, are secreted as homodimers. IL17B is expressed by T-cells and has been shown to stimulate release of TNF-alpha and IL-1beta from cells of the monocyte lineage.Synonyms: IL-17B, IL20, Interleukin-20, NIRF, Neuronal interleukin-17-related factor, ZCYTO7

Gene ID: 27190

NCBI Accession: NP_055258

UniProt: Q9UHF5