beta Defensin 1 antibody

Catalog No. ABIN181704

The Rabbit Polyclonal anti-beta Defensin 1 antibody has been validated for WB and EIA. It is suitable to detect beta Defensin 1 in samples from Human.

Quick Overview for beta Defensin 1 antibody (ABIN181704)

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Defensin 1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-beta Defensin 1 Antibody

Specificity: This antibody detects Defensin beta 1.

Purification: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human BD-1

Application Details

Application Notes: ELISA: Indirect: To detect hBD-1 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. This In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-1. Sandwich: To detect hBD-1 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanBD-1 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBD-1. Western blot: To detect hBD-1 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBD-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for beta Defensin 1

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Alternative Name: Defensin beta 1

Background: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.Synonyms: BD-1, BD1, Beta-defensin 1, DEFB1, DEFB101, HBD1, hBD-1

Gene ID: 1672

NCBI Accession: NP_005209

UniProt: P60022