beta Defensin 1 antibody (Biotin)

Catalog No. ABIN181701

The Biotin-conjugated Rabbit Polyclonal anti-beta Defensin 1 antibody (ABIN181701) specifically detects beta Defensin 1 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for beta Defensin 1 antibody (Biotin) (ABIN181701)

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Defensin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-beta Defensin 1 Antibody (Biotin)

Specificity: This antibody detects Human BD-1. Other species not tested.

Purification: Affinity Chromatography

Immunogen: E. Coli derived recombinant Human beta Defensin-1

Application Details

Application Notes: ELISA: Direct: To detect Human BD-1 (using 100 μL/well antibody solution) a concentration of0.25-1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-1. Sandwich: To detect Human BD-1 (using 100 μL/well antibody solution) a concentration of0.25-1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-Human BD-1(AP01151PU) as a capture antibody, it allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human BD-1. Western blot: To detect Human BD-1 this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant Human BD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for beta Defensin 1

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Alternative Name: Defensin beta 1

Background: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.Synonyms: BD-1, BD1, Beta-defensin 1, DEFB1, DEFB101, HBD1, hBD-1

Gene ID: 1672

NCBI Accession: NP_005209

UniProt: P60022