Betacellulin antibody (Biotin)

Catalog No. ABIN181705

This Rabbit Polyclonal antibody specifically detects Betacellulin in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Betacellulin antibody (Biotin) (ABIN181705)

Target: Betacellulin (BTC)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Betacellulin antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Betacellulin Antibody (Biotin)

Specificity: This antibody detects Betacellulin.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human Betacellulin

Application Details

Application Notes: ELISA: Direct: To detect hBetacellulin (using 100 μL/well antibody solution) a concentrationof 0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin. Sandwich: To detect hBetacellulin (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanBetacellulin as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBetacellulin. Western blot: To detect hBetacellulin this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for Betacellulin

Target: Betacellulin (BTC)

Alternative Name: Betacellulin

Background: Betacellulin is a member of the mammalian members of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This encoded protein is a ligand for the EGF receptor. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.Synonyms: BTC, Probetacellulin

Gene ID: 685

NCBI Accession: NP_001720

UniProt: P35070

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway