NOV antibody (Biotin)

Catalog No. ABIN181713

This anti-NOV antibody is a Rabbit Polyclonal antibody detecting NOV in WB and EIA. Suitable for Human.

Quick Overview for NOV antibody (Biotin) (ABIN181713)

Target: NOV (Nephroblastoma Overexpressed (NOV))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOV antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NOV Antibody (Biotin)

Specificity: This antibody detects NOV.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human NOV

Application Details

Application Notes: ELISA: Direct: To detect hNOV (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNOV. Sandwich: To detect hNOV (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanNOV as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNOV. Western blot: To detect hNOV this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hNOV is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for NOV

Target: NOV (Nephroblastoma Overexpressed (NOV))

Alternative Name: NOV

Background: CCN3 is a cysteine-rich protein that is overexpressed in avian nephroblastomas. It is a member of the CCN family of proteins that includes CTGF. These proteins are encoded by a group of genes known as immediate-early genes, so named because they are expressed after induction by growth factors or certain oncogenes. The proteins share several common structural motifs: a consensus sequence present in IGF (insulin-like growth factor)-binding proteins (the IGFBP motif), an oligomeric complex-forming domain first identified in von Willebrand factor, a binding domain to soluble and matrix molecules and a dimerization (CT) domain. All CCN family members are thought to be involved in the control of cell proliferation.Synonyms: CCN3, IGFBP9, NOVH, NovH, Protein NOV homolog

Gene ID: 4856

NCBI Accession: NP_002505

UniProt: P48745

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding