NOV antibody

Catalog No. ABIN181716

The Rabbit Polyclonal anti-NOV antibody is suitable to detect NOV in samples from Human. It has been validated for WB, IHC (p) and EIA.

Quick Overview for NOV antibody (ABIN181716)

Target: NOV (Nephroblastoma Overexpressed (NOV))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-NOV Antibody

Specificity: This antibody detects NOV.

Purification: Immunoaffinity Chromatography

Immunogen: Highly pure (> 98%) recombinant Human NOV

Application Details

Application Notes: Indirect ELISA: To detect hNOV by (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. This antibody in conjunction with compatiblesecondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinanthNOV. Sandwich ELISA: To detect hNOV (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanNOV (ABIN181714 or ABIN181713) as a detection antibody, it allows the detection of atleast 0.2-0.4 ng/well of recombinant hNOV. Western blot: To detect hNOV this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hNOV is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Formalin-Fixed, Paraffin Sections: 0.25-0.50 μg/mL with anovernight incubation at 4 °C. An HRP-labeled polymer detection system was used with aDAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended. Positive Control: Human breast invasive ductal carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for NOV

Target: NOV (Nephroblastoma Overexpressed (NOV))

Alternative Name: NOV

Background: CCN3 is a cysteine-rich protein that is overexpressed in avian nephroblastomas. It is a member of the CCN family of proteins that includes CTGF. These proteins are encoded by a group of genes known as immediate-early genes, so named because they are expressed after induction by growth factors or certain oncogenes. The proteins share several common structural motifs: a consensus sequence present in IGF (insulin-like growth factor)-binding proteins (the IGFBP motif), an oligomeric complex-forming domain first identified in von Willebrand factor, a binding domain to soluble and matrix molecules and a dimerization (CT) domain. All CCN family members are thought to be involved in the control of cell proliferation.Synonyms: CCN3, IGFBP9, NOVH, NovH, Protein NOV homolog

Gene ID: 4856

NCBI Accession: NP_002505

UniProt: P48745

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding