Thymic Stromal Lymphopoietin antibody (Biotin)

Catalog No. ABIN181717

This Rabbit Polyclonal antibody specifically detects Thymic Stromal Lymphopoietin in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Thymic Stromal Lymphopoietin antibody (Biotin) (ABIN181717)

Target: Thymic Stromal Lymphopoietin (TSLP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thymic Stromal Lymphopoietin antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Thymic Stromal Lymphopoietin Antibody (Biotin)

Specificity: This antibody detects TSLP.

Purification: Affinity chromatograophy

Immunogen: Highly pure (> 98 %) recombinant human TSLP

Application Details

Application Notes: ELISA: Direct: To detect hTSLP (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP. Sandwich: To detect hTSLP (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanTSLP as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTSLP. Western blot: To detect hTSLP this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: Synonyms: Thymic stroma-derived lymphopoietin, Thymic stromal lymphopoietin

Gene ID: 53603

NCBI Accession: NP_067342

UniProt: Q9JIE6