C19orf10 antibody (Biotin)

Catalog No. ABIN181721

The Rabbit Polyclonal anti-C19orf10 antibody is suitable to detect C19orf10 in samples from Mouse. It has been validated for WB and EIA.

Quick Overview for C19orf10 antibody (Biotin) (ABIN181721)

Target: C19orf10 (Chromosome 19 Open Reading Frame 10 (C19orf10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C19orf10 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-C19orf10 Antibody (Biotin)

Specificity: This antibody detects SF-20.

Purification: Affinity chromatograophy

Immunogen: Highly pure (> 98 %) recombinant murine SF-20

Application Details

Application Notes: ELISA: Direct: To detect mSF-20 by (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Sandwich: To detect mSF-20 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. This In conjunction with PolyclonalAnti-Murine SF-20 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/wellof recombinant mSF-20. Western blot: To detect mSF-20 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for C19orf10

Target: C19orf10 (Chromosome 19 Open Reading Frame 10 (C19orf10))

Alternative Name: SF20 / IL25

Background: Synonyms: C19orf10, IL-25, Interleukin-25, Stromal cell-derived growth factor SF20

Gene ID: 28106

NCBI Accession: NP_543027

UniProt: Q9CPT4

Pathways: ER-Nucleus Signaling