C19orf10 antibody

Catalog No. ABIN181724

This Rabbit Polyclonal antibody specifically detects C19orf10 in WB and EIA. It exhibits reactivity toward Mouse.

Quick Overview for C19orf10 antibody (ABIN181724)

Target: C19orf10 (Chromosome 19 Open Reading Frame 10 (C19orf10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C19orf10 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-C19orf10 Antibody

Specificity: This antibody detects Mouse SF-20. Other species not tested.

Purification: Immunoaffinity Chromatography employing immobilized Murine SF-20 matrix

Immunogen: Highly purified E.Coli derived recombinant Murine SF-20 (Cat.-No PA261)

Application Details

Application Notes: ELISA: Indirect: To detect mSF-20 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Sandwich: To detect mSF-20 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-MurineSF-20 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mSF-20. Western blot: To detect mSF-20 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for C19orf10

Target: C19orf10 (Chromosome 19 Open Reading Frame 10 (C19orf10))

Alternative Name: SF20 / IL25

Background: Synonyms: C19orf10, IL-25, Interleukin-25, Stromal cell-derived growth factor SF20

Gene ID: 28106

NCBI Accession: NP_543027

UniProt: Q9CPT4

Pathways: ER-Nucleus Signaling