CCL25 antibody (Biotin)

Catalog No. ABIN181760

The Rabbit Polyclonal anti-CCL25 antibody is suitable to detect CCL25 in samples from Human. It has been validated for WB and EIA.

Quick Overview for CCL25 antibody (Biotin) (ABIN181760)

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL25 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL25 Antibody (Biotin)

Specificity: This antibody detects TECK.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human TECK

Application Details

Application Notes: ELISA: Direct: To detect hTECK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK. Sandwich: To detect hTECK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanTECK as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTECK. Western blot: To detect hTECK this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for CCL25

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Alternative Name: TECK / CCL25

Background: Synonyms: C-C motif chemokine 25, Chemokine TECK, SCYA25, Small-inducible cytokine A25, Thymus-expressed chemokine

Gene ID: 6370

NCBI Accession: NP_005615

UniProt: O15444