Macrophage Scavenger Receptor 1 antibody (PE)

Catalog No. ABIN181781

The Rat Monoclonal anti-Macrophage Scavenger Receptor 1 antibody has been validated for FACS. It is suitable to detect Macrophage Scavenger Receptor 1 in samples from Mouse.

Quick Overview for Macrophage Scavenger Receptor 1 antibody (PE) (ABIN181781)

Target: Macrophage Scavenger Receptor 1 (MSR1)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Macrophage Scavenger Receptor 1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 2F8

Product Details anti-Macrophage Scavenger Receptor 1 Antibody (PE)

Specificity: This antibody recognises the murine scavenger receptor class A (SRA), type I and II, also known as CD204. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion. Recent research shows that clone 2F8 recognises an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain.

Purification: Affinity chromatography on Protein G

Immunogen: RAW264 cell line. Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Macrophage Scavenger Receptor 1

Target: Macrophage Scavenger Receptor 1 (MSR1)

Alternative Name: CD204

Background: CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions.Synonyms: MSR1, Macrophage acetylated LDL receptor I and II, Macrophage scavenger receptor types I and II, SCARA1, Scavenger receptor class A member 1

Gene ID: 20288

NCBI Accession: NP_001106797

UniProt: P30204