Interferon gamma antibody

Catalog No. ABIN181786

This Mouse Monoclonal antibody specifically detects Interferon gamma in EIA and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for Interferon gamma antibody (ABIN181786)

Target: Interferon gamma (IFNG)

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DB1

Product Details anti-Interferon gamma Antibody

Specificity: This antibody reacts with IFN-gamma. Clone DB-1 exhibits high affinity binding to the natural and recombinant forms and effectively neutralises the anti viral activity of IFN-gamma. It also blocks the induction of Ia antigen expression on murine keratinocytes. There is no cross-reaction with rodent IFN-alpha or IFN-beta or with human interferons. Clone DB-1 has also been reported for use in neutralising studies. MCA1301XZ is recommended for this purpose.

Purification: Purified

Immunogen: Recombinant rat IFN gamma.

Isotype: IgG1

Application Details

Application Notes: ELISA: 10 μg/mL - 100 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 125 mM trehalose and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma

Background: Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN

Gene ID: 15978

NCBI Accession: NP_032363

UniProt: P01580

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy