CMA1 antibody

Catalog No. ABIN181829

The Mouse Monoclonal anti-CMA1 antibody has been validated for WB, IHC (p), EIA and IHC (fro). It is suitable to detect CMA1 in samples from Human.

Quick Overview for CMA1 antibody (ABIN181829)

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CC1

Product Details anti-CMA1 Antibody

Specificity: This antibody binds to mast cell chymase, it may be used to detect mast cells in routinely fixed tissues, and has been used to study the distribution of mast cells in skin, synovium, lung and heart. Binding of this antibody to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified human skin chymase. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NSI/I.Ag4.1 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot. Immunohistochemistry on frozen sections: 1/500 - 1/1000, This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Skin
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CMA1

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Alternative Name: Mast Cell Chymase

Background: Mast Cell Chymase is an important marker of mast cells and also an important mediator of inflammation.Synonyms: Alpha-chymase, CMA1, CYH, CYM, Mast cell protease I

Gene ID: 1215

NCBI Accession: NP_001827

UniProt: P23946

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis