IL-4 antibody

Catalog No. ABIN181862

The Rat Monoclonal anti-IL-4 antibody has been validated for FACS, EIA and IHC (fro). It is suitable to detect IL-4 in samples from Mouse.

Quick Overview for IL-4 antibody (ABIN181862)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BVD6-24G2

Product Details anti-IL-4 Antibody

Specificity: This antibody is specific for Interleukin-4 (IL-4). This clone is not reported to have neutralising activity.

Purification: Affinity chromatography on Protein G

Immunogen: Recombinant murine IL-4.

Isotype: IgG1

Application Details

Application Notes: ELISA: 0.5 μg/mL - 2 μg/mL. Flow Cytometry: Membrane permeabilisation is required for this application. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: Interleukin-4 / IL4

Background: Interleukin-4 is a potent lymphoid cell growth factor, which stimulates the growth and survival of certain B and T cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra

Gene ID: 16189

NCBI Accession: NP_067258

UniProt: P07750

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation