MHC, Class I antibody (FITC)

Catalog No. ABIN181880

The Mouse Monoclonal anti-MHC, Class I antibody is suitable to detect MHC, Class I in samples from Human, Mouse, Rat, Cow, Guinea Pig, Pig and Hamster. It has been validated for FACS.

Quick Overview for MHC, Class I antibody (FITC) (ABIN181880)

Target: MHC, Class I

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC, Class I antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 2G5

Product Details anti-MHC, Class I Antibody (FITC)

Purification: Affinity chromatography on Protein G

Immunogen: Purified H-2Kb and H-2Db MHC-I molecules. Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Neat - 1/5, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: MHC Class I

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: HLA Class 1, MHC Class 1, Major Histocompatibility complex class I