MHC, Class I antibody

Catalog No. ABIN181881

The Mouse Monoclonal anti-MHC, Class I antibody (Clone 2G5) (ABIN181881) specifically detects MHC, Class I in FACS and IP. The antibody is reactive with Human, Mouse, Cow, Guinea Pig, Pig, Sheep and Hamster samples.

Quick Overview for MHC, Class I antibody (ABIN181881)

Target: MHC, Class I

Reactivity: Human, Mouse, Cow, Guinea Pig, Pig, Sheep, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC, Class I antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 2G5

Product Details anti-MHC, Class I Antibody

Purification: Affinity chromatography on Protein G

Immunogen: Purified H-2Keb and H-2Deb MHC-I molecules. Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Immunoprecipitation. Flow Cytometry: 1/10 - 1/25, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: MHC Class I

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: HLA Class 1, MHC Class 1, Major Histocompatibility complex class I