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CD8B antibody (FITC)

The Mouse Monoclonal anti-CD8B antibody has been validated for FACS. It is suitable to detect CD8B in samples from Chicken and Avian.
Catalog No. ABIN181904

Quick Overview for CD8B antibody (FITC) (ABIN181904)

Target

See all CD8B Antibodies
CD8B (CD8b Molecule (CD8B))

Reactivity

  • 76
  • 33
  • 22
  • 14
  • 14
  • 13
  • 13
  • 6
  • 5
  • 4
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Chicken, Avian

Host

  • 77
  • 60
  • 22
Mouse

Clonality

  • 80
  • 78
  • 1
Monoclonal

Conjugate

  • 81
  • 16
  • 13
  • 11
  • 5
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CD8B antibody is conjugated to FITC

Application

  • 82
  • 76
  • 39
  • 38
  • 29
  • 17
  • 17
  • 15
  • 13
  • 13
  • 8
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

01-11-39
  • Specificity

    This antibody recognises the alpha chain of the CD8 homologue, a 33-35 kDa cell surface protein. Clone 11-39 recognises all polymorphic forms of chicken CD8 alpha.

    Purification

    Affinity chromatography on Protein G

    Immunogen

    Chicken T-cells. Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.

    Isotype

    IgG1
  • Application Notes

    Flow Cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells in100l.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    CD8B (CD8b Molecule (CD8B))

    Alternative Name

    CD8 beta

    Background

    CD8 is expressed as either alpha/alpha homodimers or alpha/beta heterodimers on a subpopulation of T cells and NK cells.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain

    Gene ID

    396175

    NCBI Accession

    NP_990578

    UniProt

    Q90769

    Pathways

    TCR Signaling
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