SIRPA antibody

Catalog No. ABIN1819095

This anti-SIRPA antibody is a Mouse Monoclonal antibody detecting SIRPA in WB, FACS, IHC, IP, IHC (p) and IHC (fro). Suitable for Pig.

Quick Overview for SIRPA antibody (ABIN1819095)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BL1H7

Product Details anti-SIRPA Antibody

Specificity: Porcine CD172a, a member of the signal regulatory protein (SIRP) family. Clone BL1H7 was originally clustered as SWC3 at the Third International Swine Cluster of Differentiation Workshop (,). CD172a is expressed on monocyte derived dendritic cells (MoDCs) also conventional (cDCs), plasmacytoid (pDCs) DCs and blood DCs.(, ). Clone BL1H7 immunoprecipitated a single band of ~90-110kD from preparations of biotinylated alveolar macrophages, a result confirmed by Western blotting analysis of alveolar macrophage lysates under non reducing conditions. Has proved a useful and reliable tool for immunohistochemical analysis of routinely processed, formalin fixed, paraffin embedded porcine tissues. Aberrant expression of CD172a has been noted on porcine leukemias with blast cells co-expressing lymphocytic markers CD5 and CD25 whilst expressing the Myeloid marker CD172a in a as opposed to the more characteristic of CD172+ cells seen in normal blood PBMC.

Purification: Protein G purified

Immunogen: Porcine alveolar macrophages.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172A / SIRPA

Background: Name/Gene ID: SIRPA

Synonyms: SIRPA, CD172A, CD172a antigen, Inhibitory receptors shps-1, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, p84, SHPS-1, SHPS1, SIRP, Sirp-alpha-1, Sirp-alpha-3, Sirp-alpha1, MYD1, SIRPalpha, SIRPalpha2, BIT, MFR, MYD-1, PTPNS1, SHP substrate 1, Sirp-alpha-2

Gene ID: 140885

UniProt: P78324