Macrophages antibody

Catalog No. ABIN1819099

Product Details

Target: Macrophages

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Clone BA4D5 recognizes porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. The antigen recognized by clone BA4D5 has a broad tissue distribution and this stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-hematopoietic cells including endothelial cells. It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognized by this has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.

Purification: Protein G purified

Immunogen: Porcine alveolar macrophages.

Type of Immunogen: Cells

Clone: BA4D5

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr, IP, WB

Usage: Western blot: Under non-reducing conditions the antibody recognizes a 105 kDa protein.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details

Target: Macrophages