MAP1LC3A antibody (AA 2-15)

Catalog No. ABIN1819535

The Rabbit Polyclonal anti-MAP1LC3A antibody has been validated for WB and IF. It is suitable to detect MAP1LC3A in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Guinea Pig, Monkey, Pig and Fish.

Quick Overview for MAP1LC3A antibody (AA 2-15) (ABIN1819535)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Guinea Pig, Monkey, Pig, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-MAP1LC3A Antibody

Binding Specificity: AA 2-15

Specificity: Recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy. LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane. Recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.

Purification: Affinity purified

Immunogen: Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1, NP_852610.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Bovine, Pig, Opossum, Guinea pig, Salmon, Zebrafish (100%), Ferret, Dog, Cat, Lizard, Catfish, Stickleback, Medaka, Pufferfish (93%), Chicken, Pike (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF, WB (1:100 - 1:250)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: MAP1LC3A / LC3A

Background: Name/Gene ID: MAP1LC3A

Synonyms: MAP1LC3A, ATG8E, LC3, LC3A, MAP1BLC3, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3

Gene ID: 84557

NCBI Accession: NP_115903, NP_852610

Pathways: Autophagy