CD3 antibody (FITC)

Catalog No. ABIN1819664

The Rat Monoclonal anti-CD3 antibody has been validated for FACS. It is suitable to detect CD3 in samples from Human.

Quick Overview for CD3 antibody (FITC) (ABIN1819664)

Target: CD3

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: CD3-12

Product Details anti-CD3 Antibody (FITC)

Specificity: Raised against a peptide representing an invariant cytoplasmic sequence within the CD3epsilon chain recognizes human CD3epsilon. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, gamma, delta, zeta) that assemble and function as three pairs of dimers (epsilon-gamma, epsilon-delta, zeta-zeta,). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) , ,. CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. Ashas been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross-reactivity for the CD3 marker. ,.

Purification: Protein G purified

Immunogen: Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys). Percent identity by BLAST analysis: Chimpanzee, Gorilla, Ferret, Panda, Bovine, Dog, Cat, Opossum, Turkey, Chicken, Lizard (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:5 - 1:10)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Protect from light.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway