DC-SIGN/CD209 antibody (FITC)

Catalog No. ABIN181973

Product Details anti-DC-SIGN/CD209 Antibody (FITC)

Target: DC-SIGN/CD209 (CD209) (CD209)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DC-SIGN/CD209 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a 45 kDa C-type lectin that binds ICAM-3 which is also known as CD209. Clone MR-1 is reported to partially block the functional activity of DC-SIGN. We recommend the use of SM2219LE for this purpose.

Purification: Affinity chromatography on Protein G

Immunogen: Immature myeloid monocyte-derived dendritic cells (MDDCs). Spleen cells from immunised Balb/c mice were fused with cells of the Mouse SP2/0-Ag14 myeloma cell line.

Clone: MR-1

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Neat - 1/5, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DC-SIGN/CD209

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: CD209 / DC-SIGN (CD209 Products)

Background: CD209 is primarily expressed on a population of immature dendritic cells in peripheral tissues and on immature myeloid monocyte-derived DC's in vitro. CD209 is involved in dendritic cell (DC) migration and the initial interaction between DC's and naive T lymphocytes. CD209 also binds HIV-1 gp120 and plays a key role in the dissemination of HIV-1 by DC's.Synonyms: C-type lectin domain family 4 member L, CLEC4L, DC-SIGN1, DCSIGN, DCSIGN1, Dendritic Cell Marker, Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Gene ID: 9606

UniProt: Q9NNX6