Macrophages antibody (FITC)

Catalog No. ABIN1819801

This Mouse Monoclonal antibody specifically detects Macrophages in IHC (fro), FACS, IHC, WB and IP. It exhibits reactivity toward Pig.

Quick Overview for Macrophages antibody (FITC) (ABIN1819801)

Target: Macrophages

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Clone: BA4D5

Product Details

Specificity: Recognizes porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. The antigen recognized by the antibody has a broad tissue distribution. Clone BA4D5 stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyers patches. It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognized by this has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.

Purification: Protein G purified

Immunogen: Porcine alveolar macrophages.

Type of Immunogen: Cells

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo (1:1), IHC, IHC-Fr, IP, WB

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Protect from light.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details

Target: Macrophages

Alternative Name: Macrophage