Notch1 antibody (FITC)

Catalog No. ABIN1819821

The FITC-conjugated Mouse Monoclonal anti-Notch1 antibody (Clone mN1A) (ABIN1819821) specifically detects Notch1 in WB, IHC, FACS and IHC (fro). The antibody is reactive with Human and Mouse samples.

Quick Overview for Notch1 antibody (FITC) (ABIN1819821)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Notch1 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MN1A

Product Details anti-Notch1 Antibody (FITC)

Specificity: Recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including hematopoietic cells in mouse fetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoiesis, myelopoiesis, and neurogenesis.

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:1 - 1:10), IHC, IHC-Fr, WB

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: NOTCH1

Background: Name/Gene ID: NOTCH1

Synonyms: NOTCH1, Notch 1, TAN1

Gene ID: 4851

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development