CD163 antibody

Catalog No. ABIN1819970

The Mouse Monoclonal anti-CD163 antibody is suitable to detect CD163 in samples from Rat. It has been validated for WB, IHC, FACS, IP, IF, IHC (p) and IHC (fro).

Quick Overview for CD163 antibody (ABIN1819970)

Target: CD163

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ED2

Product Details anti-CD163 Antibody

Specificity: Recognizes the rat CD163 cell surface glycoprotein, a 175kD molecule also known as ED2. CD163 is expressed by approximately 50 % of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.* This clone may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) - refer to ref. Whiteland et al. (5.).

Purification: Protein G purified

Endotoxin Level: Low Endotoxin

Immunogen: Rat spleen cell homogenate.

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IF, IHC, IHC-Fr, IHC-P, IP, WB

Usage: The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background: Name/Gene ID: CD163

Synonyms: CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130

Gene ID: 9332