GAPDH antibody (HRP)

Catalog No. ABIN1820092

This Mouse Monoclonal antibody specifically detects GAPDH in WB, ELISA, IHC, IF, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Pig, Chicken, Dog, Monkey, Sheep, Xenopus laevis, Cat, Fish, Green Monkey and Bat.

Quick Overview for GAPDH antibody (HRP) (ABIN1820092)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Dog, Monkey, Sheep, Xenopus laevis, Cat, Fish, Green Monkey, Bat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 6C5

Product Details anti-GAPDH Antibody (HRP)

Specificity: Recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 36kD multifunctional protein whose main function is to catalyze the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin.

Purification: Protein A purified

Immunogen: Rabbit muscle GAPDH.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr, IHC-P, IP, WB (1:1000 - 1:5000)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % Thimerosal, HRP stabilizer

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Name/Gene ID: GAPDH

Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Gene ID: 2597

UniProt: P04406