ICAM1 antibody

Catalog No. ABIN182014

Product Details anti-ICAM1 Antibody

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: This antibody recognizes the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts.

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Affinity chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: Rat Ax cells (a HEV derived cell line). Remarks: Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.

Clone: 1A29

Isotype: IgG1

Application Details

Application Notes: Functional Assays. Western Blot. Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/25 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54 / ICAM1 (ICAM1 Products)

Target Type: Viral Protein

Background: CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

Gene ID: 25464

NCBI Accession: NP_037099

UniProt: Q00238

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling