CD200 antibody

Catalog No. ABIN182015

The Mouse Monoclonal anti-CD200 antibody has been validated for FACS, IHC (fro) and IP. It is suitable to detect CD200 in samples from Rat.

Quick Overview for CD200 antibody (ABIN182015)

Target: CD200

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD200 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: OX-2

Product Details anti-CD200 Antibody

Specificity: This antibody recognises the CD200 cell surface antigen, also known as OX-2. Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation.

Purification: Affinity chromatography on Protein G

Immunogen: Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD200

Target: CD200

Alternative Name: CD200

Background: CD200 is a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.Synonyms: MOX1, MOX2, My033, OX-2 membrane glycoprotein

Gene ID: 24560

NCBI Accession: NP_113706

UniProt: P04218