Sialoadhesin/CD169 antibody (FITC)

Catalog No. ABIN1820283

This anti-Sialoadhesin/CD169 antibody is a Rat Monoclonal antibody detecting Sialoadhesin/CD169 in FACS and IF. Suitable for Mouse.

Quick Overview for Sialoadhesin/CD169 antibody (FITC) (ABIN1820283)

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sialoadhesin/CD169 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MOMA-1

Product Details anti-Sialoadhesin/CD169 Antibody (FITC)

Specificity: Specific for CD169, also known as sialoadhesin. The antibody reacts with a subpopulation of mature resident tissue macrophages. No reactivity is seen with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells or blood cells. Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords but not within follicular areas or paracortex. In Peyer's patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1, although antigen expression is weaker than that seen in splenic macrophages. No MOMA-1 positive macrophages were found in the thymus. Reactivity was also negative in following organs tested so far (kidney, brain, skin). In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.

No Cross-Reactivity: Human, Rat (Rattus)

Purification: Protein G purified

Immunogen: Stromal (reticular) elements from spleen.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1), IF

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Protect from light.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details for Sialoadhesin/CD169

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Alternative Name: SIGLEC1 / CD169 / Sialoadhesin

Background: Name/Gene ID: SIGLEC1
Family: Immunoglobulin

Synonyms: SIGLEC1, CD169, CD169 antigen, SIGLEC-1, Sialoadhesin, SN, DJ1009E24.1

Gene ID: 6614