CD31 antibody (FITC)

Catalog No. ABIN182060

The Mouse Monoclonal anti-CD31 antibody has been validated for FACS. It is suitable to detect CD31 in samples from Cow and Sheep. There is 1 publication available.

Quick Overview for CD31 antibody (FITC) (ABIN182060)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: CO-3E1D4

Product Details anti-CD31 Antibody (FITC)

Cross-Reactivity (Details): Species reactivity (tested):Sheep. Reacts with Bovine and Goat.

Purification: Affinity Chromatography on Protein G.

Immunogen: Ovine leucocytes. Spleen cells from immunised BALB/c mice were fused with cells of the SP2- 0/Ag14 mouse myeloma cell line

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-CD31 antibody (FITC) (ABIN182060)

Rocheleau, Khader, Ngo, Boehnlein, McDavitt, Lattimore, Recht, McCarty, Haley: "Pilot study of novel lab methodology and testing of platelet function in adolescent women with heavy menstrual bleeding." in: Pediatric research, Vol. 83, Issue 3, pp. 693-701, (2018) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 / PECAM1

Background: CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

UniProt: P51866

Pathways: Regulation of Actin Filament Polymerization