ARNT antibody

Catalog No. ABIN1831402

This Rabbit Monoclonal antibody specifically detects ARNT in WB, IHC and IP. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for ARNT antibody (ABIN1831402)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 11C1042

Product Details anti-ARNT Antibody

Specificity: Endogenous levels of total HIF-1b/ARNT.

Purification: Purified

Immunogen: Synthetic peptide corresponding to the sequence around Ile479 of human HIF1b/ARNT.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IP, WB (1:1000)

Usage: Western Blot: Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, less than 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: ARNT / HIF-1-Beta

Background: Name/Gene ID: ARNT

Synonyms: ARNT, ARNT protein, BHLHe2, HIF-1beta, HIF1-beta, HIF1BETA, TANGO, HIF-1-beta, HIF1B

Gene ID: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect