P4HB antibody (AA 23-227)

Catalog No. ABIN1832877

This anti-P4HB antibody is a Rabbit Polyclonal antibody detecting P4HB in WB, IHC, ICC and IHC (p). Suitable for Human and Mouse.

Quick Overview for P4HB antibody (AA 23-227) (ABIN1832877)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-P4HB Antibody

Binding Specificity: AA 23-227

Specificity: Human PDI / P4HB

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 23 and 227 of PDI (SwissProt P07237). Percent identity by BLAST analysis: Human (100%), Bovine (93%), Rat (92%), Chicken (83%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:10000)

Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.0, 10 % glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Keep as concentrated solution. Avoid multiple freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI / P4HB

Background: Name/Gene ID: P4HB

Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI

Gene ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism