DYKDDDDK Tag antibody (iFluor 555)

Catalog No. ABIN1842288

The iFluor 555-conjugated Mouse Monoclonal anti-DYKDDDDK Tag antibody (ABIN1842288) specifically detects DYKDDDDK Tag.

Quick Overview for DYKDDDDK Tag antibody (iFluor 555) (ABIN1842288)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is conjugated to iFluor 555

Application: Please inquire

Product Details anti-DYKDDDDK Tag Antibody (iFluor 555)

Sequence: DYKDDDDK

Specificity: THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse recognizes N-terminal, internal and C-terminal Flag-tagged proteins.

Purification: Protein A affinity column

Immunogen: A synthetic peptide DYKDDDDK coupled to KLH

Isotype: IgG2b kappa

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The lyophilized product remains stable up to 2 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Target Type: Tag

Background: The DYKDDDK tag is a polypeptide with the amino acid sequence DYKDDDDK that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-DYKDDDK tag antibody is a useful tool for the analysis of Flag-tagged proteins.THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse is THETM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.