ALDH1A1 antibody (C-Term)

Catalog No. ABIN184581

This anti-ALDH1A1 antibody is a Goat Polyclonal antibody detecting ALDH1A1 in WB, ELISA and IF. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for ALDH1A1 antibody (C-Term) (ABIN184581)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDH1A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-ALDH1A1 Antibody

Binding Specificity: C-Term

Purpose: ALDH1A1 (C Terminus)

Sequence: EVKTVTVKIS QKNS

Specificity: This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-EVKTVTVKISQKNS

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 54.9 kDa according to NP_000680.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in IF on Human, PMID: 32157826.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ALDH1A1 antibody (ABIN184581)

Etienne, Joanne, Catelain, Riveron, Bayer, Lafable, Punzon, Blot, Agbulut, Vilquin: "Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients." in: Journal of cachexia, sarcopenia and muscle, Vol. 11, Issue 4, pp. 1047-1069, (2020) (PubMed).

Amann, Hofmann, Freudenberger, Holland-Cunz, Eichmüller, Bazhin: "Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes." in: Journal of cellular biochemistry, Vol. 113, Issue 3, pp. 792-9, (2012) (PubMed).

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1

Background: ALDH1A1, aldehyde dehydrogenase 1 family, member A1, ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1, ALDH class 1, acetaldehyde dehydrogenase 1, aldehyde dehydrogenase 1, soluble, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase, liver cytosolic

Gene ID: 216, 11668, 24188

NCBI Accession: NP_000680

Pathways: Dopaminergic Neurogenesis