ORC3 antibody (C-Term)

Catalog No. ABIN184590

This anti-ORC3 antibody is a Goat Polyclonal antibody detecting ORC3 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for ORC3 antibody (C-Term) (ABIN184590)

Target: ORC3 (Origin Recognition Complex, Subunit 3 (ORC3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ORC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ORC3 Antibody

Binding Specificity: C-Term

Purpose: ORC3L

Sequence: QKTDHVARLT WGGC

Specificity: This antibody is expected to recognize reported isoforms NP_862820.1, NP_036513.2 and NP_001184188.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QKTDHVARLTWGGC, from the C Terminus of the protein sequence according to NP_862820.1, NP_036513.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3 kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently obser

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ORC3

Target: ORC3 (Origin Recognition Complex, Subunit 3 (ORC3))

Alternative Name: ORC3

Background: ORC3L, origin recognition complex, subunit 3-like (yeast), LAT, ORC3, LATHEO, IMAGE50150, origin recognition complex, subunit 3 (yeast homolog)-like, OTTHUMP00000040611, homolog of latheo, Drosophila, RP3-486L4.1, origin recognition complex, subunit 3

Gene ID: 23595

NCBI Accession: NP_862820, NP_036513

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Feeding Behaviour, Synthesis of DNA