B-Cell Linker antibody (C-Term)

Catalog No. ABIN184602

This anti-B-Cell Linker antibody is a Goat Polyclonal antibody detecting B-Cell Linker in WB, ELISA, FACS and IF. Suitable for Human.

Quick Overview for B-Cell Linker antibody (C-Term) (ABIN184602)

Target: B-Cell Linker (BLNK)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-B-Cell Linker Antibody

Binding Specificity: C-Term

Purpose: BLNK / SLP-65

Sequence: KDSTRLKYAV KVS

Specificity: This antibody is expected to recognize all reported isoforms.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1, NP_001107566.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70-75 kDa band observed in lysates of cell line Daudi (calculated MW of 50.5 kDa according to NP_037446.1). This molecular weight is routinely observed by other sources. Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour a

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background: BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leuko

Gene ID: 29760, 17060

NCBI Accession: NP_037446, NP_001107566, NP_001245369, NP_001245370, NP_001245371

Pathways: BCR Signaling