WRNIP1 antibody (C-Term)

Catalog No. ABIN184613

The Goat Polyclonal anti-WRNIP1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect WRNIP1 in samples from Human.

Quick Overview for WRNIP1 antibody (C-Term) (ABIN184613)

Target: WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This WRNIP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-WRNIP1 Antibody

Binding Specificity: C-Term

Purpose: WHIP / WRNIP1

Sequence: EELRGVDFFK QRRC

Specificity: This antibody is expected to recognise isoform 1 (NP_064520.2) and isoform 2 (NP_569079.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence EELRGVDFFKQRRC, from the C Terminus of the protein sequence according to NP_064520.2, NP_569079.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT. Recommended concentration, 4-6 μg/mL.

Western Blot: Approx 90 kDa band observed in lysates of cell line Jurkat (calculated MW of 72.1 kDa according to NP_064520.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bish RA, Myers MP., J Biol

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for WRNIP1

Target: WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))

Alternative Name: WRNIP1

Background: WHIP, Werner helicase interacting protein, FLJ22526, putative helicase RUVBL, Werner helicase interacting protein 1, RP11-420G6.2, bA420G6.2,

Gene ID: 56897

NCBI Accession: NP_064520, NP_569079