SET/TAF-I antibody (N-Term)

Catalog No. ABIN184618

This Goat Polyclonal antibody specifically detects SET/TAF-I in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for SET/TAF-I antibody (N-Term) (ABIN184618)

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SET/TAF-I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SET/TAF-I Antibody

Binding Specificity: N-Term

Purpose: SET / I2 alpha PP2A

Sequence: SAPAAKVSKK ELNS

Specificity: This antibody is expected to recognize isoform 2 (NP_003002.2).

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SAPAAKVSKKELNS-C, from the N Terminus of the protein sequence according to NP_003002.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 7 μg/mL.

Western Blot: Approx 38 kDa band observed in lysates of Human Kidney lysates and of cell lines Daudi and Molt4 (calculated MW of 32.1 kDa according to NP_003002.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SET/TAF-I

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Alternative Name: SET

Background: SET, SET nuclear oncogene, 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA, LA-DR-associated protein II, OTTHUMP00000022286, SET translocation (myeloid leukemia-associated), Template-Activating Factor-I, chromatin remodelling factor, inhibitor of g

Gene ID: 6418

NCBI Accession: NP_003002

Pathways: Apoptosis